DNA-tethered Membrane Formation From Giant Unilamellar Vesicles
We have developed two strategies for preparing tethered lipid bilayer membrane patches on solid surfaces by DNA hybridization. In the first strategy, single-stranded DNA strands are immobilized by click chemistry to a silica surface, whose remaining surface is passivated to prevent direct assembly of a solid supported bilayer. Then giant unilamellar vesicles (GUVs) displaying the anti-sense strand based on a DNA-lipid conjugate are allowed to tether, spread, and rupture to form tethered bilayer patches. In the second strategy, a supported lipid bilayer displaying the DNA-lipid conjugate is first assembled on the surface. Then GUVs displaying the anti-sense strand are allowed to tether, spread and rupture to form tethered bilayer patches.